Calibration is used to check the exact volume of an apparatus.
APPARATUS: pipette, distilled water,beaker, weighing balance, petri dish, thermometer.
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PROCEDURES OR STEPS INVOLVES
- Measure 500ml of distilled water in a beaker.
- Take the temperature of the Distilled water.
- Pipette out 25ml of the Distilled water.
- Place the petri dish on the weighing balance and zero it.
- Put the pipettes 25ml in the petri dish and record your readings from the digital screen.
- Repeat for ten (10) values.
CALCULATION
Values gotten from the pipette
· 24.749
· 24.796
· 24.781
· 24.768
· 24.747
· 24.756
· 24.772
· 24.703
· 24.798
· 24.783
Average = 247.653
10
Average = 24.7653ml
V = WZ
W – Average weight gotten
Z – Constant = 1.0035
V = 24.7653 x 1.0035
V = 24.85ml
To calculate the percentage (%) accuracy of the pipette;
V x 100%
Vo
Vo – Volume of the pipette
24.85 x 100%
25.00
= 0.994 x 100%
= 99.4%
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